In the vapor-phase test using the closed and open containers, the response of S. zeamais and T. castaneum adults to 0.305 mg cm−3 of basil
oil, orange oil, and their components varied with the treatment method
(Fig. 1). After 24 h of exposure to basil oil, S. zeamais and T. castaneum
adults in the closed container resulted in 100% mortality (method A),
while low mortality (10–17%) was observed in the open container
(method B). Similar differences in the response of S. zeamais and
T. castaneum adults to the other tested materials in treatments A and B
were observed.