amplification of cDNA template synthesized from extracted RNA of transformed colonies (Fig. 4). The PCR product includes 986bp corresponding to a partial 5' PsaD UTR, the full ble CDS (intronless), and PsaD 3' UTR PCR transcript.
amplification of cDNA template synthesized from extracted RNA of transformed colonies (Fig. 4). The PCR product includes 986bp corresponding to a partial 5' PsaD UTR, the full ble CDS (intronless), and PsaD 3' UTR PCR transcript.