The application of B. subtilis was conducted in the first week of
May 2010 and the last week of April in 2012 near Mixquiahuala
Hidalgo, Mexico, where a history of corn smut exists. The commercial
corn hybrid AS150, known to be susceptible to head smut treated
with the insecticide Poncho 600 FS (clothianidin), was used
for the test.