2.4. Analysis of free amino acids in brown rice and GBR
Brown rice powder and GBR powder (2.5 g) were placed
in a screw test tube containing 25 ml of 70% (v/v) ethanol
solution. The mixture was vigorously mixed for 1 min at
room temperature and then centrifuged at 8000g at 4 C
for 5 min. The supernatant was filtered (No. 2, filter paper,
Whatman, Kent, England) and collected in a flask. The
same volume of 70% ethanol solution was added to theprecipitates as described above