The work presented here was carried out in the frame of the EC project “ManureEcoMine – Green fertilizer upcycling from manure”. The overall goal of the project is to demonstrate the technical feasibility of a treatment cascade, including side stream ammonia stripping as one of the major process components, to efficiently recover nutrients from digestate. The study aimed to evaluate different configurations of the stripping process for subsequent implementation in the pilot plant. One aspect of interest was to judge the effect of the initially selected stripping conditions (air stripping at 65 °C). To investigate, a series of biogas potential tests were conducted to obtain information on the residual biogas production capacity of the treated digestate. The second issue addressed was the suitability of the mentioned alternative strip gases. To gain a principle understanding of the influence of CO2 on the stripping performance, experiments with a model solution employing increasing CO2 concentrations in the strip gas were conducted. Further on experiments using simulated CHP flue gas and biogas using the model solution as well as real digestate samples were performed.