Fig. 1. Microspore development in anther culture of white calla lily (Zantedeschia aethiopica). (a) Middle part of inflorescence spadix covered with male flowers; in the hole
in the middle are the anthers removed. (b) Late-unicellular microspores isolated from anthers at initiation of culture. (c) Microspore with 7 nuclei after 2 weeks of culture.
(d) and (e) Multicellular structures after 3 weeks of culture; (d) round, enveloped in a periderm like a globular embryo, and (e) with irregular surface producing unorganized
callus. (f) Visible microspore calli on anthers after 50–70 days of culture. (g) Shoot regenerated from callus after 30 days on shoot regeneration medium. Scale bars = 1.9mm
for (a); 30m for (b)–(e); 2mm for (f); 6.7mm for (g).