Standard assay
conditions were 50 mmol L−1 Hepes buffer at different pHs (6.5 to
8.5), containing 3 mmol L−1 KCl, 50 μmol L−1 orthovanadate, 1 μg
alamethicin/μg protein and optimal concentrations of ATP and MgCl2,
with or without 1.0 μmol L−1 bafilomycin A1.