To demonstrate selectivity, chromatograms of the individual
vitamin E isomers (-, -, - and -T, and -, -, - and -T3)
and -oryzanol were compared to that of the standard mixture.
Repeatability for within-day and between-day was checked
through six consecutive injections of the sample solution. Linearity
was performed by preparing solutions of working standard mixture
at five concentration levels, i.e. 0.05–10 g mL−1 for T and T3,
and 0.5–500 g mL−1 for -oryzanol. Accuracy was determined as
the recovery of known amount of analyte spiked into placebo