Thus, the aim of the present study was to compare the skin permeation of TH as a model drug using different vesicular carriers, viz. ethosomes, binary ethosomes and transfersomes. The lipid vesicles were applied non-occlusively in vitro. Traditional liposome are not only reported to be of little value as a carrier for topical delivery (Elsayed et al., 2007b), but also, this result was certified in this study. So traditional liposomes were chosen as the control. Ethosomes, binary ethosomes and transfersomes were prepared and characterized for their size, shape, zeta-potential, and entrapment efficiency.