Extract preparation
The sample extracts, which were used in spectrophotometric
assays, were prepared in triplicate, as described previously by
Capanoglu et al. (2008). Briefly, 2 ± 0.01 g fresh weight of each
sample was extracted with 5 mL of 75% aqueous methanol containing 0.1% formic acid followed by 15 min sonication. The treated
samples were then centrifuged (Hettich Zentrifugen Universal
32R, Tuttlingen, Germany) for 10 min at 2700 g, and the supernatant was collected. Another 5 mL of 75% aqueous methanol containing 0.1% formic acid was added to the pellet,
Extract preparationThe sample extracts, which were used in spectrophotometricassays, were prepared in triplicate, as described previously byCapanoglu et al. (2008). Briefly, 2 ± 0.01 g fresh weight of eachsample was extracted with 5 mL of 75% aqueous methanol containing 0.1% formic acid followed by 15 min sonication. The treatedsamples were then centrifuged (Hettich Zentrifugen Universal32R, Tuttlingen, Germany) for 10 min at 2700 g, and the supernatant was collected. Another 5 mL of 75% aqueous methanol containing 0.1% formic acid was added to the pellet,
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