2.8 Comparison the Effect of Different Pretreatment
Methods on Ethanol Fermentation
Sugar hydrolysate from enzymatic saccharification
of pretreated sugarcane shoots and leaves was used as
substrate for ethanol production. The inoculum of S.
cerevisiae TISTR 5596 (107 cells/mL) grown in
MGYP medium, was inoculated into the conical flask
containing sugar hydrolysate. The ethanol fermentation
was conducted on orbital shaking incubator at 30 °C,
160 rpm. Aliquots (2-3 mL) were withdrawn
aseptically and assayed for ethanol concentration,
reducing sugar and cell number.