2. Materials and methods
2.1. Microorganisms and culture media
The microorganism used was S. equi subsp. zooepidemicus ATCC
35426. Stock cultures were stored at −80 ◦C in complex medium
(defined in Table 1) with 25% glycerol. The inocula were prepared
following themethodology described by Armstrong et al.[23]. Thus,
cellular suspensions of S. zooepidemicus from 16 h aged in complex