Before to do experiment the cell sheet detachment already because the density of cell is high and the time for incubate cell is long. First get the cell sheet transfer to new plate and measure 1st cell sheet. It has diameter is 0.3 mm. After that get 2nd cell sheet to overlay to 1st sheet and measure diameter. Diameter of 2nd cell sheet is 0.3 mm. In this experiment the shape of these cell sheets aren’t good shape because in method of spray the 1st cell sheet in the plate cannot set the good shape so, 2nd cell sheet cannot overlay to 1st cell sheet completely. Finally 1st and 2nd cell sheet cannot attach together because the time that use in incubate cell is too low so these cell sheets can’t attachment.