Therefore, the optimum pretreatment conditions were obtained
as follows: Before dilute NaOH (0.75 wt%) extraction at 90 C for
1 h, Fenton reagent (0.95 g/L of FeSO4 and 29.8 g/L of H2O2) pretreatment
was chosen for pretreating CS at a solid/liquid ratio of
1/20 (w/w) at 35 C for 30 min. After this combination pretreatment,
the yield of reducing sugars of FT-AE-CS could be achieved
at 70.70%. Significantly, the cost of main reagents used for pretreatment
was not so high.