Samples (2 mL) were withdrawn from each flask after every
48 h to determine the percent decolorization. These samples were
diluted appropriately and centrifuged at 12,000rpm for 15min at
room temperature. Absorbance measurements were made using
a UV/visible spectrophotometer (T60, PG Instruments, London,
UK) at respective max (Table 1). The cell path length was 1.0 cm.
In each case the absorbance value of the original uninoculated
effluent at its max was taken as reference (100% color) and the
decrease in absorbance of the inoculated effluent at the same one
wavelength was used to determine the extent of decolorization.
The most decolorized effluent was selected for optimization of
decolorization process. Blanks contained only Kirk’s basal nutrient
medium