. Isolation of bacterial strains was realized
by spreading 0.1 ml of serial dilution on Petri dishes in LB agar
medium. The various colonies were isolated in pure culture, and
then grown in 25 ml LB medium (shaking overnight at 30 C and
250 rpm). 2.5 ml volume from each culture was used to inoculate a
22.5 ml volume of BSM medium containing 300 mg l1 Sodium
Lauryl Ether Sulphate (SLES) as a sole carbon and energy source.
Four strains A4, A10, A13 and A14 possessed the highest activity of
SLES degradation were stored at 80 C on LB medium containing
15% glycerol.