Waste wheat-rye bread (after shelf-life, returns from shops) was obtained from local bakery. Whole loafs were manually cut into dices of ca. 2–4 cm size. Obtained dices were dried in a forced air oven at 40 °C for 12 h and ground in a knife mill (Rotary Mill,Brabender, Germany) with 1.5 mm internal mesh sieve. Raw material was stored in an air tight jar at room temperature until used.Starch content in waste bread was determined using Evers polarimetric method [25] and it ranged 689.13 ± 4.52 g kg of raw material dry matter. The amount of total sugars (as glucose) was measured using the DNS method [26] after mild acid hydrolysis (70 °C, 10 min) with 80 g L HCl solution (1:7.5 m/v raw material to HCl solution ratio) and it ranged 866.87 ± 2.85 g kg of raw material dry matter. Raw material moisture content was measured using WPS 50P weighing dryer (Radwag, Poland) and it ranged 41.43 ± 1.38 g kg.