Polymerase chain reactions (PCR) were
then performed using the actinobacteria-specific primers; S-C-Act-0235-a-S-20: 5
0
-GGCCTATCAGCTTG TTG-3
0
and S-C-Act-0878-a-A-19:
5
0
-CCGTACTCCCCAGGCGG GG-3
0
(Zucchi et al., 2011). The amplified 16S rRNA amplicon was sequenced by First BASE Laboratories
Pte. Ltd., and the sequences analysed using BLAST software to
determine their homologs