This work reports on supercritical CO2 extraction of lycopene from tomato peel by-product carried out with the use of indigenous oil from tomato seed obtained along with the tomato peel. The aim of this work was to determine the effect of temperature, pressure, and CO2flowrate on lycopene recovery from tomato peel in the presence of tomato seed oil as a co-solvent. We considered that the presence of tomato seed oil can improve the extraction of lycopene and have a beneficial role in the stability of lycopene due to its fatty acid content (Vasapollo et al., 2004; Lenucci et al., 2010). In addition, the effect of extraction conditions on the b-carotene extracted and the concentration of lycopene in supercritical CO2 in the presence of tomato seed oil was also investigated.Jasco, Japan); collection vials; and a wet gas meter (Sinagawa Co., Japan).