The bacterial isolate R4.4 was originally isolated from a polishing pond which is a part of a poultry processing wastewater treatment system as previously described by Ongmali et al. (2010). Stock cultures were maintained in LB agar at 4°C with a subsequent transfer to a fresh medium every month. Before use, the isolated bacterium was sub- cultured twice on LB agar, then cultivated in LB broth and incubated at 30°C for 48 hrs.