2.4.2. DPPH (1,1-diphenyl-2-picrylhydrazyl) assay For DPPH assay the modified method of Brand-Williams,
Culivier, and Berset (1995) was used. Fresh DPPH methanolic solution (A¼ 0.200 0.010) was prepared before every measurement; 2 mL DPPH solution was inserted into a plastic cuvette and absorbency in time t¼ 0 min (t0)was measured. Then a 10 mL honey sample was added (0.5 g mL1). The solution was stirred and left to stand for 20 min at laboratory temperature and then absorbency at l¼ 515 nm was measured. AOA was calculated as a decrease of absorbency from the equation