Absence of ethanol production in hypoxic
root media
No ethanol was detected in hypoxic root media
taken at different stages of 2 different cultures,
with a detection limit of 0.4 nmol/mL (data not
shown). This result is in agreement with nitrate
reduction re-oxidizing NADH that would otherwise
feed fermentation (Stoimenova et al., 2003). In the
previous study by Stoimenova et al., the excised
tobacco roots were placed under strict anoxia,
which may explain that some ethanol was produced.