3.3. Glycerol concentration
The effect of adding various concentrations of glycerol(1–8%
(v/v)) on MK-7 production was investigated.As depicted in Fig. 2,
as compared with the control media(in absence of glycerol)the
MK-7productionwasenhancedinthepresenceofdifferentgly-
cerolconcentrations.Thesedatarevealsthekeyroleofglycerolin
MK-7 biosynthesisvia Bacillus subtilisnatto metabolic pathway.
Based ontheresults,the presence of 1%(w/v)glycerol significantly
improved the MK-7 production by 18% as compared to the control
media (po0.1).However,higher glycerol concentrations showed
no improvement on enhancing the MK-7 biosynthesis.