2.2.1.4. Synthesis of chitosan from chitin by deacetylation. To the obtained chitin, 50% of NaOH was added and refluxed for 2 h at 125◦C. After completion of reaction 100 ml of water was added and made to cool down overnight. The next day it was filtered and washed well with distilled water and dried in an oven at 60-70 ◦C
In which 30 g of chitosan was obtained as a result from crabshells.