a final re-annealing at
55°C and extension at 72°C for 15 min (Gao and Gupta, 2005).
Following thermal cycling, the PCR products were visualized by
agarose gel electrophoresis and captured (Bio-red) Gel Doc. Sizes
of the amplicons were assessed with 100 bp ladder (GeNei) run in
the agarose electrophoresis.