Other methods were the
coupling of mimosine with p-nitroaniline [22] or mimosine with N-1(naphthyl)ethylenediamine
(NEDA) forming a pink-colored azodye with an absorbance of 540 nm [23], and the use
of indirect spectrophotometricity which is based on its reaction with diazotized sulfanilamide
(DZSAM).