All reactions were performed
in a Mastercycler epGradient thermal cycler (Eppendorf) with the
following protocol: initial denaturation for 2min at 94 ◦C, 32 cycles
of 1min at 94 ◦C, 1min at 54 ◦C, and 2min at 72 ◦C, with a final 7min extension at 72 ◦C. Each matK PCR reaction was performed in
a20L cocktail which included 1×Taq reaction buffer (UBI Life Sciences),
3mMMgCl2, 0.2mMdNTPs, 0.6Meach primer, 1.25U Taq
(UBI Life Sciences), and approximately 2 ng DNA.