Pre-extension evaluation of semen
Immediately after semen collection, a drop of
semen from buck’s prepuce was put on clean
warm glass slide. This drop of semen was used
to assess mass activity of the semen and the
values recorded. Volume of collected semen was
also recorded. Microscopic evaluation of semen
concentration and motility was quickly done and
the values recorded. Morphology of semen was
also studied by staining sperm cells with Eosin-
Nigrosin stain, and with Wells & Awa stain as
described by Oyeyemi et al3. In each trial, prior
to semen extension, volume, motility and
concentration of semen were quickly assessed
and the values recorded as pre-extension semen