(containing the DNA) were used as PCR templates. RAPD-PCR
analysis was performed with random primers KS, M13R2, R5 and
CC1 (Table 1). Each 25-ml PCR reaction contained 2.5 ml of 10 PCR
buffer, 3.5 mmol l1 of MgCl2, 0.2 mmol l1 of each dNTP,
0.8 mmol l1 of primer, 1 mg ml1 of BSA, 1 U of TAQ polymerase
(Invitrogen, Merelbeke, Belgium) and 2 ml of DNA.