Animal number and generalities (gender, age and breed) are described in Table 2. At the beginning of the treatments (T0), as well as at 1 (T1), 6 (T6) and 12 (follow-up-T12) months after the beginning of treatment, nasal, oropharyngeal and conjunctival swabs from each enrolled cat were combined into one pool and analyzed for FHV-1 PCR.