Material and methods.
The abdomen of each male trout was squeezed to remove the sperm which was
mixed in a single flask, then distributed into three tubes and irradiated with 110,
150 and 190y-Krads, respectively, for 20 min (10Chou.rrou,t et 1980). After irradiation,
the tubes of sperm were mixed again and kept at 4°C until fertilization. The sperm
used in the first experiment was collected from 2 males, homozygous for a dominant
depigmentation gene (yellow phenotype) serving as a paternal marker.
We used only normal pigmented females (recessive homozygous at the same
locus) which had been ovulating during the previous week. The eggs were mixed,
drained and then divided into batches ; an equal quantity of sperm (more than
1 gllegg) and an equal amount of a sperm diluent (saline solution with pH 9 buffer)
were mixed successively with the eggs which, after 15 min, were introduced into a
thermoregulated (10 oc) recirculating system for incubation. This instant was taken
as development time zero.
The thermal shocks were characterized by the three following parameters :
(i) temperature level (0), (ii) shock duration (T), (iii) pre-shock duration at 10 °C (t).
A summary of the experimental design is given in table 1.
Material and methods.
The abdomen of each male trout was squeezed to remove the sperm which was
mixed in a single flask, then distributed into three tubes and irradiated with 110,
150 and 190y-Krads, respectively, for 20 min (10Chou.rrou,t et 1980). After irradiation,
the tubes of sperm were mixed again and kept at 4°C until fertilization. The sperm
used in the first experiment was collected from 2 males, homozygous for a dominant
depigmentation gene (yellow phenotype) serving as a paternal marker.
We used only normal pigmented females (recessive homozygous at the same
locus) which had been ovulating during the previous week. The eggs were mixed,
drained and then divided into batches ; an equal quantity of sperm (more than
1 gllegg) and an equal amount of a sperm diluent (saline solution with pH 9 buffer)
were mixed successively with the eggs which, after 15 min, were introduced into a
thermoregulated (10 oc) recirculating system for incubation. This instant was taken
as development time zero.
The thermal shocks were characterized by the three following parameters :
(i) temperature level (0), (ii) shock duration (T), (iii) pre-shock duration at 10 °C (t).
A summary of the experimental design is given in table 1.
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Material and methods.
The abdomen of each male trout was squeezed to remove the sperm which was
mixed in a single flask, then distributed into three tubes and irradiated with 110,
150 and 190y-Krads, respectively, for 20 min (10Chou.rrou,t et 1980). After irradiation,
the tubes of sperm were mixed again and kept at 4°C until fertilization. The sperm
used in the first experiment was collected from 2 males, homozygous for a dominant
depigmentation gene (yellow phenotype) serving as a paternal marker.
We used only normal pigmented females (recessive homozygous at the same
locus) which had been ovulating during the previous week. The eggs were mixed,
drained and then divided into batches ; an equal quantity of sperm (more than
1 gllegg) and an equal amount of a sperm diluent (saline solution with pH 9 buffer)
were mixed successively with the eggs which, after 15 min, were introduced into a
thermoregulated (10 oc) recirculating system for incubation. This instant was taken
as development time zero.
The thermal shocks were characterized by the three following parameters :
(i) temperature level (0), (ii) shock duration (T), (iii) pre-shock duration at 10 °C (t).
A summary of the experimental design is given in table 1.
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