The obtained residues were filtered separating the liquid filtrate to soluble fibre (SF) analysis and the solid residue to insoluble fibre (IF) analysis. In both residues, SF and IF, the contents of protein and ash were calculated, and also dietary fibre content. Englyst et al. method (1994): Isolation of DF was carried out with termamyl (pH 5.2, 100 C, 10 min) followed by treatment with a mixture of pancreatin and pullulanase (pH 7.0, 50 C, 30 min).
The obtained residues were filtered separating the liquid filtrate to soluble fibre (SF) analysis and the solid residue to insoluble fibre (IF) analysis. In both residues, SF and IF, the contents of protein and ash were calculated, and also dietary fibre content. Englyst et al. method (1994): Isolation of DF was carried out with termamyl (pH 5.2, 100 C, 10 min) followed by treatment with a mixture of pancreatin and pullulanase (pH 7.0, 50 C, 30 min).
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