liquid-to-solid ratio of 30:1 (v/w), and systemic pressure of
25 kPa. After extraction, the extract was filtered. The ethanol
phase was then evaporated at 45 °C, and the water residue was
lyophilized for 48 h at −70 °C and 0.013 mbar using a vacuum
freeze drier (VirTisWizard 2.0, Gardiner, NY, USA). The extraction
was performed in triplicate.
liquid-to-solid ratio of 30:1 (v/w), and systemic pressure of25 kPa. After extraction, the extract was filtered. The ethanolphase was then evaporated at 45 °C, and the water residue waslyophilized for 48 h at −70 °C and 0.013 mbar using a vacuumfreeze drier (VirTisWizard 2.0, Gardiner, NY, USA). The extractionwas performed in triplicate.
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