The results of this study indicate that the increased concentration of PHMG derivatives in PLA and PHB films resulted in their increased bactericidal efficiency. The films containing granular PHMG polyethylene wax had bactericidal properties as set forward by standard ISO 22196, 2011. Furthermore, the results indicate inhibited biofilm formation by E. coli and S. aureus on the investigated films. The inhibition increased with increased bactericidal activity. No bactericidal properties of PLA and PHB with PHMG salt of sulfanilic acid were discovered. The samples containing PHMG granular polyethylene wax had bactericidal properties and inhibited biofilm formation. The samples containing PHMG stearate inhibited biofilm formation by Gram-negative E. coli to a certain degree and significantly inhibited biofilm formation by Grampositive S. aureus. It may therefore be concluded that the film containing higher concentrations of PHMG stearate (0.6e1%) inhibits bacterial biofilm formation despite the fact that it did not
have bactericidal properties according to ISO 22196, 20111 standards.