Extraction of rose oilThe Soxhlet extraction method was used for obtaining therose oil. Flowers were filled in a thimble placed in a cylinder. The apparatus was fitted into a flask containing 95%pure N-hexane as a solvent (Moates and Rehynolds 1991).The yield of rose petals of each treatment was used for oilextraction. The flask containing N-hexane was heateduntil boiling. Hexane was vaporized and condensed intothimble. It dissolves the compounds of petals’ volatiles. Bythis way, the organic components were obtained in theflask along with hexane. Then, the oil amounts of eachtreatment were measured in microliters by a micropipette.Statistical analysisThe LC50 value for each isolate was calculated, using Probit analysis with SPSS program (SPSS 2015). The LC50values, 95% confidence intervals (lower bound–upperbound), intercept, slope, and chi-square of each isolatewere subjected to one-way ANOVA test and means werecompared by Duncan’s test (P = 0.05). In order to correctthe mortality data in the treatments with that in thecontrol, Abbott’s formula was used (Abbott 1925). Theaphid percentage of reduction was analyzed for the fivetreatments started from day 10 after the first spray.One-way ANOVA was conducted for all parameters, andmeans were compared by Duncan’s test (P = 0.05), usingSPSS program, version 23 (SPSS 2015).