Spore chain morphology : The species with spore bearing hyphae are reported to be of (RF) Rectiflexibiles (RA) and three types: Flexible- Retinaculiaperti Open loops Characteristic of the Spira spirals (S), spore bearing hyphae and spore chains was determined by the direct examination of the culture area, microscopic standard protocol of cover slip culture technique. Plates containing sterile ISP2 medium were prepared. After solidification, medium was scooped out from the central portion of the plate by a sharp scalpel making a rectangular area. Then three sterile coverslips were placed on the hollow actinomycetes rectangular space. Slowly spores were inoculated at the edge of the coverslips touching the medium. The plates were incubated at 28t2°C for 5 days and taking out the periodically examined (Gayathri and Muralikrishnan, coverslips magnification (40X) was 2013). Adequate used to establish the presence or absence of spore chains and to observed the nature of spore chains.