The objective of this study was the quantitative determination of total As and inorganic arsenic species (As3+ and As5+) in muscle tissues of ten edible fish species, collected from arsenic-contaminated Manchar Lake, Pakistan. In this work the As3+ and As5+ was determined by ETAAS, prior to microwave assisted acid digestion. The solubilisation of the inorganic arsenic with HCl and subsequent extraction with chloroform was applied for the extraction of As3+ and As5+.