Likewise, a perfect PCR product with ~1200 bp size for ITS1 was amplified for Pseudomonas spp based on ribosomal RNA operon including the two conserved sequences of 16S and 23S rDNA (Fig.1aeb).These primer sets was reported to be highly specific and differential at the level of genus Pseudomonas with other related genera as well as within the same genus,respectively (Spilkeretal.,2004).