Sulphur dioxide was analysed in the trapping solution by using
the p-rosaniline method (Thomas et al., 1980). An aliquot of 2.5 mL
of the trapping solution was mixed with 2.5 mL of 0.1 M sodium
tetra-chloro mercurate aqueous solution. Then, 5 mL of acid
bleached p-rosaniline hydrochloride, previously filtrated with
0.45 mm Teflon® filter, and 10 mL of 0.015% (w/w) of formaldehyde
aqueous solution were added. The reaction vials were shaken and
hold for 30 min at 22 C. After that time, the absorbance at 550 nm
was measured using a Spectrophotometer UV-1700 Pharmaspec
from Shimadzu Iberica (Madrid, Spain). A blank sample using
2.5 mL of 0.01Msodium hydroxide solution instead of the trapping
solution was used as reference. Three replicates were carried out.
Sulphur dioxide was analysed in the trapping solution by usingthe p-rosaniline method (Thomas et al., 1980). An aliquot of 2.5 mLof the trapping solution was mixed with 2.5 mL of 0.1 M sodiumtetra-chloro mercurate aqueous solution. Then, 5 mL of acidbleached p-rosaniline hydrochloride, previously filtrated with0.45 mm Teflon® filter, and 10 mL of 0.015% (w/w) of formaldehydeaqueous solution were added. The reaction vials were shaken andhold for 30 min at 22 C. After that time, the absorbance at 550 nmwas measured using a Spectrophotometer UV-1700 Pharmaspecfrom Shimadzu Iberica (Madrid, Spain). A blank sample using2.5 mL of 0.01Msodium hydroxide solution instead of the trappingsolution was used as reference. Three replicates were carried out.
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