Ca2+-dependent active
force (Factive), Ca2+-independent passive force (Fpassive),
and Ca2+-sensitivity of force production were determined
in single, mechanically isolated, permeabilized cardiomyocytes.
Stress- and ovariectomy-induced alterations in myofilament
proteins (myosin-binding protein C [MyBP-C], troponin
I [TnI], and titin) were analyzed by sodium dodecyl
sulfate gel electrophoresis using protein and phosphoprotein
stainings.