The measurement of the H2O2 content and the determination of lipid peroxidation were performed in the same extraction; 300 mg of powdered leaves was homogenised in 2 mL of 0.1% (w/v) trichloroacetic acid (TCA) and centrifuged at 10,000 g for 10 min at 4 C (Alexieva et al., 2001). The assay consisted of a mixture of