Elution was performed in a single step from 0% to 100% AEC-B (50 mM Tris–HCl, 1 MNaCl, pH 8.0). For column recovery the column was washed with 5 CV of a 1 M NaOH/1 M NaCl solution at an average linear flow rate of 33.6 cm h1.
Elution was performed in a single step from 0% to100% AEC-B (50 mM Tris–HCl, 1 MNaCl, pH 8.0). For column recoverythe column was washed with 5 CV of a 1 M NaOH/1 M NaClsolution at an average linear flow rate of 33.6 cm h1.