For the conservation of high value but neglected medicinal plant (Asparagus racemosus) of Nepal
an effort has been made to multiply its number rapidly using tissue culture technique. The plant has been
successfully multiplied. For the multiplication, all possible explants including callus have been used and the
callus and shoot explants (shoot tips and nodes) played significant role. Mainly four parameters: callus, buds,
shoots and roots inductions were studied. NAA singly played a good role in all parameters except bud
induction. Similarly, BAP played good roles in shoot and bud inductions, whereas combinations of NAA and
BAP at various levels were found to be effective in almost all cases. The multiplied adventitious shoots were
successfully rooted (in vitro and in vivo) using NAA, acclimatized and transferred to natural conditions.