Dissolve 2.5 g anhydrous sodium carbonate, 2 g sodium bicarbonate, 2.5 g potassium sodium tartrate and 20 g anhydrous sodium sulphate in 80 mL water and make up to 100 mL.
Dissolve 15 g copper sulphate in a small volume of distilled water. Add one drop of sulphuric acid and make up to 100 mL.
Mix 4 mL of B and 96 mL of solution A before use.
Arsenomolybdate reagent: Dissolve 2.5 g ammonium molybdate in 45 mL water. Add 2.5 mL sulphuric acid and mix well. Then add 0.3 g disodium hydrogen arsenate dissolved in 25 mL water. Mix well and incubate at 37°C for 24–48 hours.
Standard glucose solution: Stock: 100 mg in 100 mL distilled water.
Working standard: 10 mL of stock diluted to 100 mL with distilled water [100 µg/mL]