In this regard, the paper presents an automated method, which counts the RBCs present in a blood sample. The first
step is to convert the image from RGB color space to gray scale image then, this gray scale image is converted into binary
image by applying Otsu thresholding method. Then, all the non-peripheral holes are filled in this binary image followed
by smoothing. The next step is to fill the peripheral holes and finally, a hole less image is produced. From this hole
less image, each cell is extracted and categorized. Finally, the total number of cells present in this image is counted.
The performance of the proposed approach, along with a comparison with related method, is demonstrated on a set of real images.