Three tomato varieties (Lycopersicon esculentum Mill.) viz. BARI tomato-11, BARI tomato-2 and BARI
tomato-3 released from Bangladesh Agriculture Research Institute (BARI) were characterized by karyotype
analysis and RAPD markers. BARI tomato-11 and BARI tomato-2 were found to possess 2n = 24
metacentric chromosomes. On the other hand, 2n = 25 metacentric chromosomes were observed in BARI
tomato-3 revealing a trisomic variety. The karyotype of the three varieties was very symmetric and each of
the varieties showed specific and unique RAPD fingerprinting. Therefore, based on karyotype and RAPD
analysis the three tomato varieties could be characterized.