Food and beverage samples (5 g, or 5 ml) in duplicate were
inoculated with a cocktail of vegetative cells or endospore
suspensions (3.0–3.5 106 CFU/ml) from strains CECT 43 and 49
prepared as described above, and then supplemented with enterocin AS-48 at desired final concentrations (1.75–7 mg per g or ml of
sample). Untreated controls and bacteriocin-treated samples were
placed in an incubation chamber with a controlled temperature of
45 C (Memmert, Schwabach, Germany). At desired intervals, peas and corn samples were homogenised with a Stomacher 80