When inoculating mycelia of R. solani on horsebean leaves,
many speckles formed quickly on the leaves (Fig. 3). After 5 days,
the entire leaves became necrotic, brown and died. However, when
spraying the CHM1 culture filtrate with R. solani on the leaves, the
leaves remained green and healthy, indicating that CHM1 has
the capacity for protecting horsebean against infection by R. solani.
The disease index for R. solani alone was 71.67% but it decreased to
26.67% when CHM1 and R. solani were inoculated together with
62.79% protection effect