A commercially prepared M. oleifera food supplement containing
powdered leaves and marketed by Bari-Frank Moringa Nigeria Limited
was used in this study. They were packaged in containers with
each container weighing 153.91 g of leaves, which were dissolved in
1 L of methanol and shaken vigorously. The sample was then filtered
after 3 days using a Buckner funnel and Wartman No. 1 filter paper.
The filtrated sample was exposed to atmospheric air to evaporate the
methanol. The extract was further concentrated using a water bath.
The weight of the extract was 14.7 g. The graded solutions of the
extract were prepared and used for the experiments.