5. Conclusions
Resistance of bacterial strains to various types of environmental factors, mainly their formation and consequences, are still under investigations of numerous scientists worldwide. Therefore, some new concepts and methodologies are still looked for. In this study, we did an overview of the effects of some metal ions on S. aureus with the emphasis on the occurrence of the resistance to the presence of these ions. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals. The analysis was done by resistance curve and viability, enzyme activity, metallothionein(as metal binding protein), protein profile and detection of mutants by RFLP. Resistance and viability results show that studies metals could be divided to three groups with different resistant mechanism, (i) zinc, (ii) cadmium, lead and copper and (iii) silver. Changes Both in biochemical and protein profiles of heavy metal resistant S. aureus indicate that the observed changes in biochemical pro-files are caused mainly by modification in protein expression and minor by heavy metals caused enzymes inhibition or activation. Changes in protein expression are caused by the great presence of point mutations by heavy metal-resistant S. aureus.
5. Conclusions
Resistance of bacterial strains to various types of environmental factors, mainly their formation and consequences, are still under investigations of numerous scientists worldwide. Therefore, some new concepts and methodologies are still looked for. In this study, we did an overview of the effects of some metal ions on S. aureus with the emphasis on the occurrence of the resistance to the presence of these ions. Our results describe the possible option for the analysis of S. aureus resistant strains and may thus serve as a support for monitoring of changes in genetic information caused by the forming of resistance to heavy metals. The analysis was done by resistance curve and viability, enzyme activity, metallothionein(as metal binding protein), protein profile and detection of mutants by RFLP. Resistance and viability results show that studies metals could be divided to three groups with different resistant mechanism, (i) zinc, (ii) cadmium, lead and copper and (iii) silver. Changes Both in biochemical and protein profiles of heavy metal resistant S. aureus indicate that the observed changes in biochemical pro-files are caused mainly by modification in protein expression and minor by heavy metals caused enzymes inhibition or activation. Changes in protein expression are caused by the great presence of point mutations by heavy metal-resistant S. aureus.
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